Intermediate
Part:BBa_I725022:Design
Designed by: Wayne Materi Group: iGEM07_Alberta (2007-10-23)
RBS + Enoyl-coa hydratase
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 181
Illegal AgeI site found at 312 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
Because B0034 is too small to cutout from a gel, this part was cloned into the plasmid containing B0034.
Source
RBS from B0034, Enoyl-coa hydratase is E. coli codon optimized from C. acetobutylicum