Intermediate

Part:BBa_I725022:Design

Designed by: Wayne Materi   Group: iGEM07_Alberta   (2007-10-23)


RBS + Enoyl-coa hydratase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 181
    Illegal AgeI site found at 312
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Because B0034 is too small to cutout from a gel, this part was cloned into the plasmid containing B0034.


Source

RBS from B0034, Enoyl-coa hydratase is E. coli codon optimized from C. acetobutylicum

References